Information

Links

• A Series of video tutorials on how to navigate through the flowjo software and analyse the FCS files created by the FACS lab.  https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-with-flowjo/66
• A Brief introduction to flow cytometry. http://www.abcam.com/protocols/introduction-to-flow-cytometry
• Courses provided by the manufacturer of the sorters and analysers used at the Institute of Genetics and Cancer. http://www.bdbiosciences.com/eu/s/training/e-learning
• An introduction to flow cytometry. http://flowbook.denovosoftware.com/
• A central location for articles, resources, protocols and videos. http://www.abcam.com/tag/flow%20cyt

• Protocols

• Abcam have a directory of protocols categorised by application and research area. http://www.abcam.com/index.html?pageconfig=popular_protocols

Downloadable Resources

Hints and Tips

• Please bring negative controls as these help us to set up your experiment.
• If samples are clumpy, please filter before bringing samples as this may clog the machines.
• Bring collection tubes already prepared with collection media/PBS/FCS
• Label collection tubes and sample tubes appropriately
• If sample sorting is for RNA or DNA then we would recommend to bring the samples and collection tubes on ice. We are able to maintain a low temperature when the sample is being run and also we are able to cool the collection chamber.

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