DNA Sequencing Facility

Sequencing facility, data and analysis.

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DNA sequencing facility

An in house DNA sequencing facility providing Sanger and 2nd generation sequencing to all IGMM researchers and their collaborators, operated by two experienced sequencing technologists.

  • Sample numbers ranging from single tube through to 384 well plates can be submitted to the facility.  
  • Provides an automated PCR purification service directly before Sanger Sequencing.
  • Plasmid mini-prep extraction and isolation service processed by liquid handling robots.  
  • DNA and RNA quality and quantitation is available via Tapestation/Bioanalyzer.
  • Human Cell Line Authentication using STR profiling.

Instrumentation

 
Sanger Instruments Capillaries
3130xl 16
3730xl 96
Liquid handling instruments Pipette channels
BioMek NX (x2) 1, 8 and 96
DNA/RNA Qualititative and Quantitative Analysers Chip Type and Sensitivity range
Agilent 2100 DNA 1000; DNA High Sensitivity
  RNA Nano; RNA Pico
Agilent 4200 TapeStation DNA 1000; DNA 5000; RNA

People

 
Stewart McKay Technical Services Manager
Stephen Brown Facility Manager
Jeffrey Joseph Sequencing Technologist

Contact

For more information on the services provided within the facility, please contact:

sequence@igc.ed.ac.uk

 

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DNA sequencing image